Gogoi, D K and Dekaboruah, H P and Saikia, R and Bora, T C
(2008)
Optimization of process parameters for improved production of bioactive metabolite by a novel endophytic fungus Fusarium sp. DF2 isolated from Taxus wallichiana of North East India.
World Journal of Microbiology and Biotechnology, 24 (1).
pp. 79-87.
Abstract
An endophytic fungus having antifungal and antibacterial
properties was isolated from Taxus wallichiana of Arunachal
Pradesh, India. On the basis of morphological and molecular
characteristics, the fungus was identified as Fusarium sp. and
designated as DF2. The fungus was optimized for growth and
maximum production of the antimicrobial agent. Media with
5% leaf extract (w/v) supplemented with 0.1% dextrose as
carbon and yeast extract as nitrogen source favored the
growth with temperature optimum 25 ± 2°C and pH 6.
Incubation period of 10 days was observed optimum for growth
and production of antimicrobial agent. Phenylalanine and
dextrose enriched basal medium promoted the antimicrobial
agent production, whereas methionine amended in combination
with glucose promoted higher biomass accumulation. The TLC
purified active compound with UV λ-max 270 nm in ethyl
acetate has got the lowest minimum inhibitory concentration
(MIC) against Bacillus subtilis, Staphylococcus aureus and
Escherichia coli and highest against Pseudomonas aeruginosa.
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