SAIKIA , R and Gogoi, D K and Mazumder, S and Yadav, A and Sarma, R K and Bora, T C and Gogoi , B K
(2011)
Brevibacillus laterosporus strain BPM3,a potential biocontrol agent isolated from a natural hot water spring of Assam, India.
Microbiological Research, 166 (3).
pp. 216-225.
ISSN 0944-5013
Abstract
A bacterial strain designated as BPM3 isolated from mud of a natural hot water
spring of Nambar Wild Life Sanctuary, Assam, India, strongly inhibited growth of
phytopathogenic fungi (Fusarium oxysporum f. sp. ciceri, F. semitectum, Magnaporthe
grisea and Rhizoctonia oryzae) and gram-positive bacterium (Staphylococcus
aureus). The maximum growth and antagonistic activity was recorded at 30 %E6C, pH
8.5 when starch and peptone were amended as carbon and nitrogen sources, respectively.
In greenhouse experiment, this bacterium (BPM3) suppressed blast disease
of rice by 30 1467% and protected the weight loss by 35 1456.5%. The maximum disease
protection (67%) and weight loss protection (56.5%) were recorded when the
bacterium was applied before 2 days of the pathogen inoculation. Antifungal and
antibacterial compounds were isolated from the bacterium which also inhibited the
growth of these targeted pathogens. The compounds were purified and on spectroscopic
analysis of a purified fraction having Rf 0.22 which showed strong antifungal
and antibacterial activity indicated the presence of C 14H, carbonyl group, dimethyl
group, 14CH2 and methyl group. The bacterium was characterized by morphological,
biochemical and molecular approaches and confirmed that the strain BPM3 is
Brevibacillus laterosporus.
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