Bhau, B S and Medhi, K and Das, A P and SAIKIA , S P and Neog, K and Choudhury, S N
(2009)
Analysis of Genetic Diversity of Persea bombycina
‘‘Som’’ Using RAPD-Based Molecular Markers.
Biochem Genet, 47.
pp. 486-497.
Abstract
The utility of RAPD markers in assessing genetic diversity and phenetic
relationships in Persea bombycina, a major tree species for golden silk (muga)
production, was investigated using 48 genotypes from northeast India. Thirteen
RAPD primer combinations generated 93 bands. On average, seven RAPD fragments
were amplified per reaction. In a UPGMA phenetic dendrogram based on
Jaccard’s coefficient, the P. bombycina accessions showed a high level of genetic
variation, as indicated by genetic similarity. The grouping in the phenogram was
highly consistent, as indicated by high values of cophenetic correlation and high
bootstrap values at the key nodes. The accessions were scattered on a plot derived
from principal correspondence analysis. The study concluded that the high level of
genetic diversity in the P. bombycina accessions may be attributed to the species’
outcrossing nature. This study may be useful in identifying diverse genetic stocks of
P. bombycina, which may then be conserved on a priority basis.
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