KHAN , RAJU and Solanki, Pratima R. and Kaushik, Ajeet and Singh, S P and Ahmed, Sharif and Malhotra, B D (2009) Cholesterol biosensor based on electrochemically prepared polyaniline conducting polymer film in presence of a nonionic surfactant. J Polymer Research, 16. pp. 363-373.

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Cholesterol biosensor has been fabricated by covalently coupling cholesterol oxidase (ChOx) via glutaraldehyde onto electrochemically prepared polyaniline film in presence of TritonX-100 [4-(1,1,3,3-tetramethylbutyl) phenyl polyethylene glycol], a non-ionic surfactant onto indium-tin-oxide (ITO) glass substrate. These ChOx/PANITX- 100/ITO bioelectrodes have been characterized using Fourier transform infrared (FTIR) spectroscopy, cyclic voltammetry (CV) and scanning electron microscopy (SEM) techniques. The results of response measurements carried out on ChOx/PANI-TX-100/ITO bioelectrodes using amperometric and photometric techniques, reveal detection limit as 5 mg/dl, linearity from 5 to 400 mg/dl of cholesterol and sensitivity as 131 μA/(mg/dl cm−2). These biosensing electrodes are thermally stable up to 65 °C, can be used about 20 times and have a shelf-life of about 10 weeks when stored at 4 °C. Attempts have also been made to utilize the ChOx/PANI-TX-100/ITO bioelectrodes for estimation of free cholesterol concentration in serum samples.

Item Type: Article
Subjects: Chemistry > Analytical Chemistry
Depositing User: Dr. PK Barooah
Date Deposited: 29 Dec 2011 07:12
Last Modified: 29 Dec 2011 07:12

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