BHAU, B S and NEGI , S K and JINDAL , S K and SINGH , M and LAKSHMIKUMARAN , M (2007) Assessing genetic diversity of Tecomella undulata (Sm) - an endangered tree species using amplified fragment length polymorphisms-based molecular markers. Current Science, 93 (1). pp. 67-72.

[img]
Preview
PDF (2504) - Published Version
Download (65Kb) | Preview

Abstract

Tecomella undulata is an important agro-forestry tree in the western parts of India, which has been included in the list of endangered plant species due to over exploitation. There is an urgent need to formulate appropriate conservation and breeding strategies to save this versatile tree. Quantification of genetic diversity in the existing populations of T. undulata prevalent in India, is an essential first step towards this objective. The present work was undertaken to investigate the utility of amplified fragment length polymorphism (AFLP) markers in the assessment of genetic diversity and phenetic relationship among 42 T. undulata plants collected from different regions of India. Eight AFLP primer combinations generated a total of 338 bands across the 42 T. undulata accessions screened. On an average, 42 AFLP fragments were amplified per reaction. Of the total 338 AFLP fragments, 71.7% was polymorphic across all the accessions. From the AFLP data, a similarity matrix based on Jaccard's coefficient was calculated which was used for constructing a phenetic dendrogram using UPGMA analysis. T. undulata accessions represented high levels of genetic variation as indicated by GS values ranging from 0.721 to 0.83, at which 42 T. undulata accessions grouped together. The pattern of grouping in the phenogram was highly consistent as indicated by high values of cophenetic correlation (r = 0.87, P = 1) as also by high bootstrap values at the key nodes. Principal correspondence analysis also Cited By since 1996 This article has been cited 0 times in Scopus. Inform me when this document is cited in Scopus: E-mail Alert Find related documents In Scopus based on On the Web based on title Document Type: Article View references (31) references authors keywords authors keywords Page 1 of 5 Scopus - Current Science: Assessing genetic diversity of Tecomella undulata (Sm.) - An endangered... 02/04/2008 http://www.scopus.com/scopus/record/display.url?view=basic&eid=2-s2.0-3 revealed that T. undulata accessions were scattered on the plot. Based on our study, it may be concluded that there is a high level of genetic diversity in T. undulata accessions analysed, which may be attributed to its out-crossing nature. Our study may be useful in identifying diverse genetic stocks of T. undulata, which may then be conserved on priority basis. Author Keywords AFLP; Conservation; Genetic diversity; Molecular makers; Tecomella undulata References (31) view in table layout Select: Page 1. (1982) The Wealth of India, 10, pp. 134-136. Publications and Information Directorate, CSIR, New Delhi, Anon 2. Jindal, S.K., Kackar, N.L., Solanki, K.R. Evaluation of Tecomella undulata (Sm.) SEEM germplasm from western Rajasthan in situ (1985) Trans. Indian Soc. Desert Technol. Univ. Cent. Desert Stud, 10, pp. 33-37. 3. Jindal, S.K., Solanki, K.R., Kackar, N.L. Phenology and breeding systems of Rohida (Tecomella undulata (Sm.) SEEM) (1985) Indian J. For, 8, pp. 317-320. 4. Jindal, S.K., Kackar, N.L., Solanki, K.R. Germplasm collection and genetic variability in Rohida (Tecomella undulata (Sm.) Seem) in western Rajasthan (1987) Indian J. For, 10, pp. 52-55. 5. Rao, A., Kiran-Bala, V., Lahiri, A.N., Bala, K. Influence of trees on microorganisms of aridisol and its fertility (1989) Indian For, 115, pp. 680-683. 6. Azam, M.M. Anti-HIV agents and other compounds from Tecomella undulata (1999) Orient. J. Chem, 15, pp. 375-377. Cited 2 times. 7. Dushyent, G., Bohra, A. Toxic effects of various plant part extracts on the causal organism of typhoid fever (2000) Curr. Sci, 78, pp. 780-781. Cited 4 times. 8. Ahmad, F., Alam Khan, R., Rasheed, S. Preliminary screening of methanolic extracts of Celastrus paniculatus and Tecomella undulata for analgesic and antiinflammatory activities (1994) Journal of Ethnopharmacology, 42 (3), pp. 193-198. Cited 12 times. doi: 10.1016/0378-8741(94)90085-X 9. Jain, S.K., Rao, R.R. An assessment of threatened plants of India (1983) Botanical Survey of India, Howrah, p. 334. Page 2 of 5 Scopus - Curr

Item Type: Article
Subjects: Biology > Plant Science
Divisions: UNSPECIFIED
Depositing User: Dr. PK Barooah
Date Deposited: 03 Jan 2012 06:38
Last Modified: 03 Jan 2012 06:38
URI: http://neist.csircentral.net/id/eprint/265

Actions (login required)

View Item View Item