HAZARIKA , S and GOSWAMI , P and DUTTA , N N and HAZARIKA , A K (2002) Elyyal oleate synthesis by Porcine pancreatic lypase in organic solvent. Chemical Engineering Journal, 85 (1). pp. 61-68.

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The Porcine pancreatic lipase catalysed esterification of oleic acid with ethanol was studied in 10 different solvents with constant initial water content in the reaction mixture. The initial rates of the esterification reaction were attempted to correlate with such solvent properties as hydrophobicity (log P), water solubility (Sw), dielectric constant, electron pair acceptance and donation index (expressed as EN T + DNN), polarisability etc. While significantly good linear correlations with log P and log Sw were obtained, the correlations with the other properties were found to be inferior. The kinetics of the reactions was found to conform to the so-called Ping-Pong-Bi-Bi model with ethanol inhibition effect and the estimated model parameters exhibited statistically significant correlation with log P consistent to its correlation with the initial rate. Assuming that organic solvents do not interfere with the lipase–substrate binding process nor with the catalytic mechanism, the contribution of substrate–solvent interactions to enzyme kinetics was accounted for by replacing the substrate concentrations of the intrinsic kinetic equations by thermodynamic activities. The values of the corrected intrinsic parameters (Km, ksp) and the maximal rate (Vmax) were found to be nearly equal for all the media

Item Type: Article
Subjects: Engineering > Chemical Engineering
Depositing User: Dr. PK Barooah
Date Deposited: 11 Jan 2012 04:18
Last Modified: 11 Jan 2012 04:18
URI: http://neist.csircentral.net/id/eprint/356

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