Thakur, D and Roy, M K and Bora, T C
(2009)
Expandase-like activity mediated cell-free conversion
of ampicillin to cephalexin by Streptomyces sp. DRS I.
Biotechnology Letters, 31.
pp. 1059-1064.
Abstract
Cell-free extracts of Streptomyces sp.
DRS I converted ampicillin to cephalexin, presumably
due to the activity of the enzyme, expandase. The
extract was fractionated and characterized by colorimetric
and chromatographic measurements coupled
with disc-agar diffusion bioassay against an ampicillinresistant,
cephalexin-sensitive E. coli strain. Though
expandase could not be identified, the presence of
a hitherto unreported expandase in Streptomyces sp.
DRS I is suggested.
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