CHOUDHURY , A and GUHA , A and YADAV , A and KUMARI , J and UNNI , B G and ROY , M K (2004) Induced immunity in Antheraea assama Ww larvae against flacherie causing Pseudomonas aeruginosa AC-3. Experimental Parasitology, 106. pp. 75-84.

Preview

PDF (1148) - Published Version Download (151Kb) | Preview

Abstract

This study reports for the first time the induction of immunity in Antheraea assama Ww larvae against bacterial flacherie. In silkworms group of disease caused by bacteria are collectively called 1Cflacherie. 1D This refers to the flaccid condition of the larvae due to the infections of bacterial strains pathogenic to muga silkworm. Antibacterial activity against pathogenic Pseudomonas aeruginosa AC-3 causing flacherie, was induced by injection of heat-killed cells of the same strain. Experiments on larval survivability and viable cell count revealed peak immune response on third day. Comparison of the amount of food ingested, excreta produced and larval weight of the salineinjected control, live bacteria-challenged larvae and heat-killed bacteria-injected larvae 1C(vaccinated) 1D confirmed the development of immunity against bacterial infection in the 1Cvaccinated 1D set. The haemolymph of A. assama larvae was analyzed for proteins associated with bacterial infection. Out of the total 32 detected proteins, eleven (A1 132, A15 1320, A22 1323, and A29) were constitutively synthesized in both the control and live bacteria-injected larvae. Four inducible proteins A4, A9 1310, and A21 were detected in the haemolymph of the live bacteria-injected larvae. Synthesis of rest of the proteins varied between the control and their live bacteria-injected counterparts. General protein profile of 1Cvaccinated 1D larvae injected with live bacteria were found to be similar to that of the saline-injected control. Author Keywords: Antheraea assama; Flacherie; Pseudomonas aeruginosa; Immunity; Haemolymph proteins Article Outline 1. Introduction 2. Materials and methods 2.1. Rearing of silkworms 2.2. Induction of disease symptoms and immunity 2.3. Determination of time period for peak immune response 2.4. Feeding efficiency 2.5. Haemolymph preparation 2.6. Comparative antibacterial activity of immune and normal haemolymph 2.7. SDS 13PAGE of haemolymph 3. Results 4. Discussion Acknowledgements References

Item Type:	Article
Subjects:	Biology > Biotechnology
Divisions:	UNSPECIFIED
Depositing User:	Dr. PK Barooah
Date Deposited:	06 Jan 2012 06:12
Last Modified:	06 Jan 2012 06:12
URI:	http://neist.csircentral.net/id/eprint/315

Actions (login required)

View Item